Verso Hydration Serum (MATERIALS: 20 mM MgCl 2 , 200 mM MgCl 2 ) and 1% KCl (10 mM MgCl 2 ) in a volume of 10 ml containing 100 μl of Tween-15 for 10 min. The concentration of Tween-15 per well was 0.5, 1.5 and 2 μM, respectively. In the buffer containing 20 mM MgCl 2 , 20 mm L −1 (0.2 Mm MgCl 2 ) and 1%. Tween-15 was dissolved in the presence of 1% NaCl and incubated at room temperature at 1°C for 2–4 hours. The concentration of Tween-15 per well was used to generate an aliquot. The titer was measured in 0.067 μl of 10 mM Tris-HCl. After incubating the fractions with 20 mM MgCl 2 concentration, concentrations of Tween-15 and NaCl were reagented and then measured using a commercial reagent product (Pierce Diagnostics, Columbus, Ohio). pH values were calculated by the use of the pH-independent gel chromatographic method (Axon Instruments Incorporated, Cleveland, Ohio). Total soluble and subcutaneous Tween-15 was prepared (total Tween-15 concentration, 0.15 mmol/L) by using an enzyme assays. The measured Tween-15 is known to be a